Tuesday, October 29, 2013 -
11:00am to 12:00pm

2013-2014 CREOSA Seminar Series

"Single Molecule Imaging in Vivo Determines Transcriptional RNA Processing Dynamics" 


Mr. Matthew Ferguson

Systems Biology of Gene Expression, Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Bethesda, MD


This event takes place in Rm 600, 6th flr of the Wm C Jason Library at 11 a.m..


The synthesis and splicing of pre-mRNA to form completed transcripts requires coordination between two large multi-subunit complexes (the RNA polymerase and the spliceosome). Using dual-color single molecule RNA imaging in living human cells, we observe kinetic competition during the transcription cycle which results in both co- and post- transcriptional splicing of pre-mRNA. By combining transcription site fluctuation analysis and single RNA imaging we construct a model of RNA synthesis and processing. We see that the majority of pre-mRNAs are spliced after release, while diffusing away from the site of transcription. By single particle tracking, we measure the distribution of pre-mRNA around the site of transcription. By Raster Image Correlation Spectroscopy (RICS), we measure the diffusion coefficient of pre-mRNA and determine that pre-mRNA is spliced within 5 seconds of cleavage from the transcription site suggesting that intron removal is more efficient after cleavage and release of nascent RNA. We further investigate the role of splicing factors and mutations on the transcription and splicing dynamics and also the localization of pre-mRNA.

See attached flier.

This event is part of the 2013-2014 OSCAR Seminar Series. Contact Ms. Jackie Jones for more information about this program:

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