Publications 1. J.L. Lee and R.E. Levin. Detection of 5 CFU/g of Escherichia coli O157:H7 on lettuce using activated charcoal and real-time     PCR without enrichment. 2011. Food Microbiology. 2011. 28: 562-567. 2. J.L. Lee and R.E. Levin. Selective detection of mixed bacterial survivors from fish fillets after freezing and thawing by ethidium bromide monoazide real-time PCR. Food Biotechnology. 2010. 24: 270-281. 3. J.L. Lee and R.E. Levin. Discrimination of viable and dead Vibrio vulnificus after refrigerated and frozen storage using EMA, sodium deoxycholate and real-time PCR. Journal of Microbiological Methods. 2009. 79: 184-188. 4. J.L. Lee and R.E. Levin. Improved detection of g-irradiated Vibrio vulnificus after heat and cold shock treatment by using ethidium monoazide real-time PCR. Food Science and Biotechnology. 2009. 18: 788-792. 5. J.L. Lee and R.E. Levin. Detection of Vibrio vulnificus before and after g-irradiation in quahog clams (Mercenaria mercenaria) tissue using real-time polymerase chain reaction. Food Biotechnology. 2009. 23: 121-132. 6. J.L. Lee and R.E. Levin. A comparative study of the ability of EMA and PMA to distinguish viable from heat killed mixed bacterial flora from fish fillets. Journal of Microbiological Methods. 2009. 76: 93-96. 7. J.L. Lee and H.Y. Kim. Expression and iron storage properties of recombinant human ferritins in Escherichia coli:Relevance for functional ferritins in food systems. Food Biotechnology. 2008. 22: 140-149. 8. J.L. Lee and R.E. Levin. New approach for discrimination of g-irradiated Vibrio vulnificus by using Real-time polymerase chain reaction. Journal of Microbiological Methods. 2008. 73: 1-6. 9. J.L. Lee and R.E. Levin. and H. Y. Kim. Improved co-expression and multi-assembly properties of recombinant human ferritins subunit in Escherichia coli. J. Microbiol. Biotechnol. 2008. 18: 926-932. 10. J.L. Lee and R.E. Levin. Discrimination of g-irradiated Vibrio vulnificus by using ethidium monoazide real-time polymerase chain reaction. Journal of Applied Microbiology. 2008. 104: 728-734. 11. J.L. Lee and R.E. Levin. Quantification of total viable bacteria on fish fillets by using ethidium monoazide Real-time polymerase chain reaction. International Journal of Food Microbiology. 2007. 118: 312-317. 12. J.L. Lee., C.S. Park., and H.Y. Kim. Functional assembly of recombinant human ferritin subunits in Pichia pastoris. J. Microbiol. Biotechnol. 2007. 17: 1695-1699. 13. J.L. Lee and R.E. Levin. Rapid quantification of total bacteria on cod fillets by using real-time PCR. Journal of Fisheries Sciences. 2007. 1: 58-67. 14. J.L. Lee and R.E. Levin. Selection of universal primer for quantification of total bacteria associated with fish fillets. Food Biotechnology. 2006. 20: 275- 285. 15. J.L. Lee and R.E. Levin. Direct application of the polymerase chain reaction for quantification of total bacteria on fish fillets. Food Biotechnology. 2006. 20: 287-298. 16. J.L. Lee and R.E. Levin. Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction. Journal of Microbiological Methods. 2006. 67: 456- 462. 17. J. L. Lee, S.N. Yang, C. S. Park, D. Jeoung, and H. Y. Kim.  Purification and its glycosylation pattern of recombinant L-      ferritin in Pichia pastoris. J. Microbiol. Biotechnol. 2004. 14: 68-73. 18. J. L. Lee, H. S. Song, H, J. Kim, J. H. Park., D. K. Chung., C. S. Park., D. Jeoung., and H. Y. Kim. Functional expression and production of human H-ferritin in Pichia pastoris. Biotechnology Letters. 2003. 25:1019-1023. 19. S. N. Yang, H. S. Song., J. L. Lee and H. Y. Kim. Selection of enhanced iron uptake yeast mutants by EMS mutagenesis. Kor. J. Microbiol. Biotechnol. 2003. 31: 435-438. 20. J. L. Lee., J. H. Park., I. S. Jung., D, Jeoung., S. K. Cho., H. Y. Kim. Purification and characterization of the recombinant      human Iron Regulatory Protein 2 expressed in Escherichia. coli. Agric. Chem.Biotechnol. 2002. 45:117-120. 21. J. L. Lee, J. H. Park and H. Y. Kim.  Identification of differentially expressed genes using RNA differential display. Agric. Chem.Biotechnol. 1999. 42: 123-127. 22. H. Y. Kim, I. S. Jung, and J. L. Lee. Regulation of ferritin synthesis in 5’-untranslated region by Iron Responsive Element.  Agric. Chem.Biotechnol. 1998. 41: 224-227.     Presentations 1.  J. L. Lee. Microbial evaluation of the freshness of cod fillet using real-time PCR for quantification of bacteria on        fish fillets. UKC Annual meeting. (2009, Raleigh, USA). 2. J. L. Lee and R. E. Levin. Selective detection of mixed bacterial survivors from fish fillets after freezing and thawing by ethidium bromide monoazide real-time PCR. IFT Annual meeting. (2009, Anaheim, USA). 3. J. L. Lee and R. E. Levin. Discrimination of gamma (r)-irradiated & non irradiated Vibrio vulnificus in quahog clams (Mercenaria mercenaria) using real-time PCR. IFT Annual meeting. (2008, New Orleans, USA). 4. J. L. Lee and R. E. Levin. Discrimination of g-irradiated & non irradiated Vibrio vulnificus in quahog clams (Mercenaria mercenaria) using Real-Time Polymerase Chain Reaction. Annual Atlantic Fisheries Technology Conference. (2007, Portland, USA). 5. J. L. Lee and R. E. Levin. Discrimination of g-irradiated & non r-irradiated Vibrio vulnificus using Real-Time Polymerase Chain Reaction. IFT Annual meeting. (2007, Chicago. USA). 6. J. L. Lee. Use of ethidium bromide monoazide (EMA) for quantification of viable and dead bacteria from fish fillets by PCR and real-time PCR. Cambridge Healthtech Institute’s nucleic acid-based technologies conference. (2007, Baltimore, USA). 7. J. L. Lee and R. E. Levin. Quantification of total viable bacteria on fish fillets by using PCR and EMA real-time        PCR. Trans-Atlantic Fisheries Technology Conference.(2006,Quebec, Canada). 8. J. L. Lee and R. E. Levin. Rapid quantification of viable total bacteria on fish fillets by using ethidium bromide     monoazide real-time PCR. IFT Annual meeting. (2006, Orlando. USA). 9. J.L. Lee, and H. Y. Kim. Expression of recombinant human H-ferritin in Pichia pastoris. CHI Conference (2004,     San Diego, USA). 10. J.L. Lee, and H. Y. Kim. Functional expression of human L-ferritin in Pichia pastoris. Annual- Meeting of the Korean Society for Microbiology and Biotechnology, Bio Exhibition &International Symposium. (2003, Muju, Korea). 11. J.L. Lee, and H. Y. Kim. Expression of human H-and L-ferritin in Pichia pastoris. Annual Meeting of the Korean society for microbiology and biotechnology. (2001, Fall, Sang-Rok, Korea). 12. J.L. Lee, and H. Y. Kim. The constriction of bicistronic expression vector for studying human ferritin         heteropolymers. Annual Meeting of the Korean Society for Applied Biological Chemistry. (2000, Spring, Young-       Nam National Univ, Korea). 13. J.L. Lee, and H. Y. Kim. Overexpression of recombinant ferritins in E. coli. Annual Meeting of the Korean Society of Food Science & Technology. (1999, Fall, Kyung-Hee Univ, Korea). 14. J.L. Lee, and H. Y. Kim. Characterization of the recombinant Human H-L-Ferritin. Annual Meeting of the Korean Society for Applied Biological Chemistry. (1999, Spring, Kang-Won National Univ, Korea). 15. J.L. Lee, J. H. Park, and H. Y. Identification of differentially expressed genes using RNA differential display. Annual Meeting of the Korean Society for Biochemistry and Molecular Biology. (1999, Spring, A-Ju Univ, Korea). 16. J.L. Lee and H. Y. Kim. Expression and purification of human Ferritin in Escherichia. coli. Annual Meeting of       the Korean Society for Biochemistry and Molecular Biology. (1999, Spring, A-Ju Univ, Korea). 17. J.L. Lee and H. Y. Kim. Characterization of Iron-regulated protein in iron metabolism. Annual Meeting of the       Korean Society for Molecular and Cellular Biology. (1998, Seoul National Univ, Korea). 18. J.L. Lee and H. Y. Kim. Characterization of Nramp2 as an iron transport protein. Annual Meeting of the Korean       Society for Biochemistry and Molecular Biology. (1998, Fall, KAIST, Korea). 19. J.L. Lee and H. Y. Kim. Structure and function of the iron responsive element. Annual Meeting of the Korean       Society for Biochemistry and Molecular Biology. (1998, Spring, Korea Univ, Korea). 20. J.L. Lee and H. Y. Kim. Binding characterization of iron regulatory protein to RNA ligand. Annual Meeting of       the Korean Society for Applied Biological Chemistry. (1997, Seoul National Univ, Korea).  

Dahlia Jackson-O'Brien, PhD Assistant Professor and Small Ruminant Specialist Department of Agriculture and Natural Resources College of Agriculture & Related Sciences Delaware State University 1200 N. DuPont Hwy Dover, Delaware 19901 302-857-6490 djjackson@desu.edu     Dr. Dahlia Jackson-O’Brien is an Assistant Professor and Small Ruminant Specialist in the Department of Agriculture and Natural Resources at Delaware State University. Dr. O'Brien received her PhD from the University of Maryland Eastern Shore in December of 2005. Prior to her position at Delaware State University, Dr. O'Brien served as the Acting Director of the Coastal Ecology Teaching and Research Center in Berlin, MD. As a graduate student, Dr. O'Brien was involved with projects examining the influence of melengestrol acetate or temporary kid removal in inducing breeding during the late non-breeding or postpartum period in goats; the correlation of milk and blood leptin in does and growth of their offspring; the use of poultry litter as a nitrogen source in small ruminants; anthelmintic resistance in a Maryland small ruminant flock; and the influence of breed and nutrition on growth, parasite resistance, carcass traits and meat quality of crossbred Katahdin lambs. Dr. O'Brien's current research interest include characterizing levels of anthelmintic resistance (AR) in gastrointestinal nematodes (GIN) of small ruminants in the Mid-Atlantic U.S., evaluating the efficacy of natural/alternative dewormers and genetics in parasite control, evaluating the use of goats as a biological control for invasive weeds, and identifying methods to increase sheep and goat production to satisfy increasing market demands (synchronization and timed-artificial insemination).   Research   Research Interests: My current research interests include characterizing levels of anthelmintic resistance (AR) in gastrointestinal nematodes (GIN) of small ruminants in the Mid-Atlantic U.S., evaluating the efficacy of natural/alternative dewormers and genetics in parasite control, evaluating the use of goats as a biological control for invasive weeds, and identifying methods to increase sheep and goat production to satisfy increasing market demands (synchronization and timed-artificial insemination).   Graduate Students' Research Elizabeth Crook (2008 - 2010; currently at Virginia Maryland Regional College of Veterinary Medicine) Study: Characterization of Anthelmintic Resistance in Small Ruminant (USDA Capacity Building Grant) Graduate students Jenna Warren and Kwame Matthews conducting fecal egg counts in laboratory.  Jenna Warren (2009 - present) Study: Use of goats to control roadside invasive weeds in Delaware   Kwame Matthews (2010 - present) Study: Natural dewormers and genetics in controlling internal parasites in small ruminants     Undergraduate Summer Research   Characterization of Anthelmintic Resistance in Small Ruminant Gastrointestinal Nematodes (GIN) in the Mid-Atlantic States Out of Season Breeding Garlic as a natural plant dewormer in goats (NESARE project) Anthelmintic Resistance in a Small Goat Herd in Delaware Progesterone Measurement to Determine Estrus Activity in Does at Hickory Hill Comparison of the Efficiency of Two- and Three- Chambered Slides on Determining Fecal Egg Counts in Goats Mating behavior of bucks during the non-breeding season after estrus synchronization protocols in meat does An Observation of the Internal Parasitic Variations Found Using the Modified McMaster Technique, in Relation to Domesticated Goat Kids   Research Position: There are no research positions available at the moment.